Plasmid

Part:BBa_K4213060:Design

Designed by: Eleftheria Lakaki   Group: iGEM22_Thessaly   (2022-09-28)


pDGB3omega1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 366
    Illegal NheI site found at 3745
    Illegal PstI site found at 405
    Illegal NotI site found at 1620
    Illegal NotI site found at 3152
    Illegal NotI site found at 4442
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 366
    Illegal BamHI site found at 5
    Illegal BamHI site found at 387
    Illegal BamHI site found at 626
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
    Illegal NgoMIV site found at 1065
    Illegal NgoMIV site found at 1239
    Illegal NgoMIV site found at 1289
    Illegal NgoMIV site found at 1293
    Illegal NgoMIV site found at 1320
    Illegal NgoMIV site found at 1819
    Illegal NgoMIV site found at 2177
    Illegal AgeI site found at 4239
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 11
    Illegal BsaI.rc site found at 620
    Illegal SapI site found at 4833
    Illegal SapI.rc site found at 4370


Design Notes

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Source

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References